Under the Risk of COVID-19 Epidemic: A Study on the Influence of Life Attitudes, Leisure Sports Values, and Workplace Risk Perceptions on Urban Development and Public Well-Being

This study examined the impact of attitudes toward life, recreational sports values, and workplace risk perceptions on urban development and public well-being under the risk of the COVID-19 epidemic in China. A mixed-method research study was conducted, and 2400 valid questionnaires were collected via purposeful sampling. The questionnaires were analyzed using the SPSS 26.0 statistical software and validated with basic statistical methods and Pearson's correlation analysis. Semi-structured interviews were then conducted to collect the opinions of 12 respondents on the questionnaire results, including academics, foreign entrepreneurs and employees, local entrepreneurs and employees, and official institutions. Finally, all the data collected were discussed via triangulation analysis. The survey found that different cities' social and industrial development and job market needs lead to different attitudes toward life, leisure values, and perceptions of job-seeking and employment risks. The smoother a person's growing-up background, the better their learning history, the better their learning and working experience, and the richer their life experience;consequently, positive attitudes toward life, correct leisure values, and positive attitudes toward job hunting and employment can be cultivated. In addition, people with positive attitudes toward life, leisure values, and job hunting and employment can improve their city's economy and environment for sustainable development, thereby improving their quality of life and increasing their well-being.

[Improvement of China's legal system for public health emergency management from the perspective of lifecycle management].

A crucial lesson gained through the pandemic preparedness and response to COVID-19 is that all measures for epidemic control must be law-based. The legal system is related not only to public health emergency management per se but also to all aspects of the institutional supporting system throughout the lifecycle. Based on the lifecycle emergency management model, this article analyses the problems of the current legal system and the potential solutions. It is suggested that the lifecycle emergency management model shall be followed to establish a more comprehensive public health legal system and to gather the intelligence and consensus of experts with different expertise, including epidemiologists, sociologists, economists, jurist and others, which will collaboratively promote the science-based legislation in the field of epidemic preparedness and response for the establishment of a comprehensive legal system for public health emergency management and with Chinese characteristics.

Evaluation and Prediction of Coupling Coordinated Development of Compound System of Key State-owned Forest Region in Daxing'anling, Heilongjiang Province

Coordination is an important part of the new development philosophy. Promoting the coordinated development is the main goal of deepening the reform and development in state-owned forest region. Aiming to provide scientific basis and theoretical supports for promoting the continuous deepening of reform of state-owned forest region and realizing comprehensive and high-quality coordinated development, the key state-owned forest region in Daxing'anling, Heilongjiang Province was chosen as the research object. A compound system covering ecological conservation, industrial development, enterprise management, well-being of the people and support capability was constructed. The coupling coordination model was used to quantitatively evaluate the coupling coordination status of the compound system from 2000 to 2020. The Grey Markov model was used to predict the trend of coupling coordination development in this compound system from 2021 to 2022. Results show that, after 21-year of transformation and development, the development index of each subsystem of state-owned forest region in Daxing'anling, Heilongjiang Province has been changed, however, the process were different among subsystems. The growth rates of the subsystems of well-being of the people and resource conservation have been high, while the subsystems of enterprise management and the support capability have been lagged dramatically behind. The development stage of coupling coordination of the compound system has changed from misalignment to coordination, nevertheless, the coordinated development level was regressive in recent years due to certain factors such as policy, COVID-19, etc. It is predicted that by 2022, the development stage of coupling coordination of compound system will be recovered to the benign coordinated development type, however, there is still a big gap before it reaches the high-quality coordinated development type. It is suggested that the existing support policies and inputs should be kept stable, moreover, the enterprise management and support capability should be strengthened, in order to promote the stable and high-quality coupling coordinated development in the key state-owned forest regions in Daxing'anling, Heilongjiang Province. © 2022, China Environmental Science Press. All rights reserved.

Tourists’ travel intention: revisiting the TPB model with age and perceived risk as moderator and attitude as mediator

Purpose: This study aims to understand tourists’ travel intention during the COVID-19 pandemic by using the theory of planned behavior (TPB) with additional causal links and moderators. The three research objectives of this study are to explore basic causality, mediating effects of attitude and moderating effects of age and perceived risk, of the extended TPB model, respectively. Design/methodology/approach: An online survey was conducted in Taiwan during the COVID-19 pandemic. In total, the survey generated 351 responses. The structural model analysis was performed to test the cause-and-effect relationships in the traditional TPB model and the mediating role of an individual’s attitude regarding travel during the COVID-19 pandemic. The SmartPLS multi-group analysis procedure was applied to test the moderation effects of age and perceived risk. Findings: It is found that the perceived behavioral control is the main contributor of travel intention for the traditional TPB while attitude partially mediates the relationship between subjective norm and travel intention for the test of mediation. Also, moderation tests confirm that both age and perceived risk show significant moderating effect only on subjective norm to travel intention relationship. Originality/value: The novelty of this research is that this study proposed and verified the mediating influence of attitude and two additional paths, the moderating influence of age and perceived risk, which deepened the understanding of the TPB model and the impact of COVID-19 on travel intention. Because TPB model is context-dependent, using one mediating and two moderating variables allows the study to understand how TPB functions in a different situation. © 2022, Emerald Publishing Limited.

A highly sensitive cell-based luciferase assay for high-throughput automated screening of SARS-CoV-2 nsp5/3CLpro inhibitors.

Effective drugs against SARS-CoV-2 are urgently needed to treat severe cases of infection and for prophylactic use. The main viral protease (nsp5 or 3CLpro) represents an attractive and possibly broad-spectrum target for drug development as it is essential to the virus life cycle and highly conserved among betacoronaviruses. Sensitive and efficient high-throughput screening methods are key for drug discovery. Here we report the development of a gain-of-signal, highly sensitive cell-based luciferase assay to monitor SARS-CoV-2 nsp5 activity and show that it is suitable for the screening of compounds in a 384-well format. A benefit of miniaturisation and automation is that screening can be performed in parallel on a wild-type and a catalytically inactive nsp5, which improves the selectivity of the assay. We performed molecular docking-based screening on a set of 14,468 compounds from an in-house chemical database, selected 359 candidate nsp5 inhibitors and tested them experimentally. We identified two molecules which show anti-nsp5 activity, both in our cell-based assay and in vitro on purified nsp5 protein, and inhibit SARS-CoV-2 replication in A549-ACE2 cells with EC50 values in the 4-8 µM range. The here described high-throughput-compatible assay will allow the screening of large-scale compound libraries for SARS-CoV-2 nsp5 inhibitors. Moreover, we provide evidence that this assay can be adapted to other coronaviruses and viruses which rely on a viral protease.